Ontology highlight
ABSTRACT:
SUBMITTER: Fukui K
PROVIDER: S-EPMC3634412 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Fukui Kenji K Bessho Yoshitaka Y Shimada Atsuhiro A Yokoyama Shigeyuki S Kuramitsu Seiki S
International journal of molecular sciences 20130321 3
Polymerase chain reaction (PCR)-related technologies are hampered mainly by two types of error: nonspecific amplification and DNA polymerase-generated mutations. Here, we report that both errors can be suppressed by the addition of a DNA mismatch-recognizing protein, MutS, from a thermophilic bacterium. Although it had been expected that MutS has a potential to suppress polymerase-generated mutations, we unexpectedly found that it also reduced nonspecific amplification. On the basis of this find ...[more]