Magnetic barcode assay for genetic detection of pathogens.
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ABSTRACT:
SUBMITTER: Liong M
PROVIDER: S-EPMC3635151 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Magnetic barcode assay for genetic detection of pathogens.
Liong Monty M Hoang Anh N AN Chung Jaehoon J Gural Nil N Ford Christopher B CB Min Changwook C Shah Rupal R RR Ahmad Rushdy R Fernandez-Suarez Marta M Fortune Sarah M SM Toner Mehmet M Lee Hakho H Weissleder Ralph R
Nature communications 20130101
The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magn ...[more]
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