Ontology highlight
ABSTRACT:
SUBMITTER: Yaroslavsky AI
PROVIDER: S-EPMC3636569 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Yaroslavsky Anastasia I AI Smolina Irina V IV
Chemistry & biology 20130301 3
We present an approach for fluorescent in situ detection of short, single-copy sequences within genomic DNA in human cells. The single-copy sensitivity and single-base specificity of our method is achieved due to the combination of three components. First, a peptide nucleic acid (PNA) probe locally opens a chosen target site, which allows a padlock DNA probe to access the site and become ligated. Second, rolling circle amplification (RCA) generates thousands of single-stranded copies of the targ ...[more]