Unknown

Dataset Information

0

Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae.


ABSTRACT: Subunit F of V-ATPases is proposed to undergo structural alterations during catalysis and reversible dissociation from the V1VO complex. Recently, we determined the low resolution structure of F from Saccharomyces cerevisiae V-ATPase, showing an N-terminal egg shape, connected to a C-terminal hook-like segment via a linker region. To understand the mechanistic role of subunit F of S. cerevisiae V-ATPase, composed of 118 amino acids, the crystal structure of the major part of F, F(1-94), was solved at 2.3 ? resolution. The structural features were confirmed by solution NMR spectroscopy using the entire F subunit. The eukaryotic F subunit consists of the N-terminal F(1-94) domain with four-parallel ?-strands, which are intermittently surrounded by four ?-helices, and the C terminus, including the ?5-helix encompassing residues 103 to 113. Two loops (26)GQITPETQEK(35) and (60)ERDDI(64) are described to be essential in mechanistic processes of the V-ATPase enzyme. The (26)GQITPETQEK(35) loop becomes exposed when fitted into the recently determined EM structure of the yeast V1VO-ATPase. A mechanism is proposed in which the (26)GQITPETQEK(35) loop of subunit F and the flexible C-terminal domain of subunit H move in proximity, leading to an inhibitory effect of ATPase activity in V1. Subunits D and F are demonstrated to interact with subunit d. Together with NMR dynamics, the role of subunit F has been discussed in the light of its interactions in the processes of reversible disassembly and ATP hydrolysis of V-ATPases by transmitting movements of subunit d and H of the VO and V1 sector, respectively.

SUBMITTER: Basak S 

PROVIDER: S-EPMC3636880 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae.

Basak Sandip S   Lim Jackwee J   Manimekalai Malathy Sony Subramanian MS   Balakrishna Asha Manikkoth AM   Grüber Gerhard G  

The Journal of biological chemistry 20130308 17


Subunit F of V-ATPases is proposed to undergo structural alterations during catalysis and reversible dissociation from the V1VO complex. Recently, we determined the low resolution structure of F from Saccharomyces cerevisiae V-ATPase, showing an N-terminal egg shape, connected to a C-terminal hook-like segment via a linker region. To understand the mechanistic role of subunit F of S. cerevisiae V-ATPase, composed of 118 amino acids, the crystal structure of the major part of F, F(1-94), was solv  ...[more]

Similar Datasets

| S-EPMC34635 | biostudies-literature
| S-EPMC4909252 | biostudies-literature
| S-EPMC4318993 | biostudies-literature
| S-EPMC3433196 | biostudies-literature
| S-EPMC5287370 | biostudies-literature
| S-EPMC3164464 | biostudies-literature
| S-EPMC4089900 | biostudies-literature
| S-EPMC2781686 | biostudies-literature
| S-EPMC6496941 | biostudies-literature
| S-EPMC2330193 | biostudies-literature