Proteomic analysis of vitreous biopsy techniques.
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ABSTRACT:
SUBMITTER: Skeie JM
PROVIDER: S-EPMC3637028 | biostudies-literature | 2012 Nov-Dec
REPOSITORIES: biostudies-literature
Publications
Proteomic analysis of vitreous biopsy techniques.
Skeie Jessica M JM Brown Eric N EN Martinez Harryl D HD Russell Stephen R SR Birkholz Emily S ES Folk James C JC Boldt H Culver HC Gehrs Karen M KM Stone Edwin M EM Wright Michael E ME Mahajan Vinit B VB
Retina (Philadelphia, Pa.) 20121101 10
<h4>Purpose</h4>To compare vitreous biopsy methods using analysis platforms used in proteomics biomarker discovery.<h4>Methods</h4>Vitreous biopsies from 10 eyes were collected sequentially using a 23-gauge needle and a 23-gauge vitreous cutter instrument. Paired specimens were evaluated by UV absorbance spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and liquid chromatography tandem mass spectrometry (LC-MS/MS).<h4>Results</h4>The total protein concentration obtained wi ...[more]
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