Dataset Information

Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D? or D?supplementation.

ABSTRACT: BACKGROUND: It is not known whether genetic variation in the vitamin D binding protein (DBP) influences 25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of total 25(OH)D, 25(OH)D? and 25(OH)D? in a Thai cohort, according to type of vitamin D supplement (vitamin D? or D?) and DBP genotype, after receiving vitamin D? or D? for 3?months. METHODS: Thirty-nine healthy subjects completed the study. All subjects received 400 ?IU of either vitamin D? or D?, plus a calcium supplement, every day for 3?months. Total serum 25(OH)D, 25(OH)D? and 25(OH)D? were measured by LC-MS/MS. Individual genotyping of rs4588 in the DBP gene was performed using real-time PCR. RESULTS: Vitamin D? supplementation of 400 ?IU/d increased 25(OH)D? significantly (+16.2?±?4.2?nmol/L, p <0.001). Vitamin D? (400?IU/d) caused increased 25(OH)D? levels (+22.0?±?2.11?nmol/L, p <0.001), together with a decrease of 25(OH)D? (-14.2?±?2.0?nmol/L, p <0.001). At 3?month, subjects in vitamin D3 group tended to have higher total 25(OH)D levels than those in vitamin D? (67.8?±?3.9 vs. 61.0?±?3.0?nmol/L; p?=?0.08). Subjects were then classified into two subgroups: homozygous for the DBP rs4588 C allele (CC), and the rest (CA or AA). With D? supplementation, subjects with CA or AA alleles had significantly less increase in 25(OH)D? and total 25(OH)D when compared with those with the CC allele. However, no difference was found when the supplement was vitamin D?. CONCLUSION: Genetic variation in DBP (rs4588 SNP) influences responsiveness to vitamin D? but not vitamin D?.

SUBMITTER: Nimitphong H

PROVIDER: S-EPMC3637219 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D₃ or D₂supplementation.

Nimitphong Hataikarn H Saetung Sunee S Chanprasertyotin Suwannee S Chailurkit La-Or LO Ongphiphadhanakul Boonsong B

Nutrition journal 20130404

<h4>Background</h4>It is not known whether genetic variation in the vitamin D binding protein (DBP) influences 25-hydroxyvitamin D levels [25(OH)D] after vitamin D supplementation. We aimed to investigate the changes of total 25(OH)D, 25(OH)D₃ and 25(OH)D₂ in a Thai cohort, according to type of vitamin D supplement (vitamin D₃ or D₂) and DBP genotype, after receiving vitamin D₃ or D₂ for 3 months.<h4>Methods</h4>Thirty-nine healthy subjects completed the study. All subjects received 400 IU of e ...[more]

PMID: 23556437

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Project description:Serum free 25-hydroxyvitamin D (25(OH)D) rather than total 25(OH)D may better indicate vitamin D status during pregnancy given the pregnancy-associated increase in serum vitamin D binding protein (DBP) concentration. Our aims were to assess changes in DBP and free 25(OH)D across gestation and to determine whether free compared with total 25(OH)D more strongly correlates with markers of vitamin D and calcium metabolism during pregnancy. This ancillary study included 58 pregnant adolescents (53% African American, 47% White) who completed a vitamin D3 supplementation study in Rochester, NY. Blood was collected at entry, mid-study, and delivery (median 17, 29, and 40 weeks' gestation). Mixed-effects regression was used to test for differences in DBP, directly measured free 25(OH)D, and other serum markers by study visit and race. Free and total 25(OH)D were evaluated in relation to serum PTH, 1,25(OH)2D, 24,25(OH)2D, and calcium. The mean DBP concentration was above nonpregnant reference values at entry and increased across gestation (P < 0.0001). Total 25(OH)D explained most of the variance in free 25(OH)D (r ≥ 0.67; P < 0.0001). Holding total 25(OH)D constant, each 100 mg/L increase in DBP was associated with a 0.4 pg/mL decrease in free 25(OH)D (P < 0.01). The percent free 25(OH)D was inversely related to both DBP and total 25(OH)D at each visit. Regardless of race or visit, total 25(OH)D was a stronger correlate of PTH, 1,25(OH)2D, and 24,25(OH)2D, and neither total nor free 25(OH)D was related to serum calcium. African Americans had lower total 25(OH)D (P < 0.0001), but free 25(OH)D did not significantly differ by race (P = 0.2). In pregnant adolescents, DBP concentration was elevated and inversely associated with percent free 25(OH)D, but measured free 25(OH)D provided no advantage over total 25(OH)D as a predictor of PTH, 1,25(OH)2D, 24,25(OH)2D, or calcium. The clinical relevance of the small racial difference in percent free 25(OH)D requires further investigation.

Dataset Information

Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D? or D?supplementation.

Publications

Changes in circulating 25-hydroxyvitamin D according to vitamin D binding protein genotypes after vitamin D₃ or D₂supplementation.

Similar Datasets

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