Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model.

ABSTRACT: The transformation of normal cells to malignant, metastatic tumor cells is a multistep process caused by the sequential acquirement of genetic changes. To identify these changes, we compared the transcriptomes and levels and distribution of proteins in a four-stage cell model of isogenically matched normal, immortalized, transformed, and metastatic human cells, using deep transcriptome sequencing and immunofluorescence microscopy. The data show that ∼6% (n = 1,357) of the human protein-coding genes are differentially expressed across the stages in the model. Interestingly, the majority of these genes are down-regulated, linking malignant transformation to dedifferentiation. The up-regulated genes are mainly components that control cellular proliferation, whereas the down-regulated genes co

SUBMITTER: Danielsson F 

PROVIDER: S-EPMC3637701 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature