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Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori.

ABSTRACT: Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only. Transgenic animals show female-specific lethality during embryonic and early larval stages, leading to male-only cocoons. This transgene-based female-specific lethal system not only has wide application in sericulture, but also has great potential in lepidopteran pest control.

SUBMITTER: Tan A 

PROVIDER: S-EPMC3637792 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori.

Tan Anjiang A   Fu Guoliang G   Jin Li L   Guo Qiuhong Q   Li Zhiqian Z   Niu Baolong B   Meng Zhiqi Z   Morrison Neil I NI   Alphey Luke L   Huang Yongping Y  

Proceedings of the National Academy of Sciences of the United States of America 20130408 17

Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative  ...[more]

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