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Preparation and application of a 'clickable' acceptor for enzymatic synthesis of heparin oligosaccharides.

ABSTRACT: A 'clickable' disaccharide was prepared by treating the aldehyde precursor with hydroxylamine, followed by the catalytic hydrogenation and diazotransfer reaction. This disaccharide was successfully applied to the elongation of the backbone construction of ultralow molecular weight (ULMW) heparins using two bacterial glycosyl transferases, N-acetyl glucosaminyl transferase from Escherichia coli K5 (KfiA) and heparosan synthase-2 (pmHS2) from Pasteurella multocida.

SUBMITTER: Cai C

PROVIDER: S-EPMC3638765 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Preparation and application of a 'clickable' acceptor for enzymatic synthesis of heparin oligosaccharides.

Cai Chao C Edgar Kristi K Liu Jian J Linhardt Robert J RJ

Carbohydrate research 20130301

A 'clickable' disaccharide was prepared by treating the aldehyde precursor with hydroxylamine, followed by the catalytic hydrogenation and diazotransfer reaction. This disaccharide was successfully applied to the elongation of the backbone construction of ultralow molecular weight (ULMW) heparins using two bacterial glycosyl transferases, N-acetyl glucosaminyl transferase from Escherichia coli K5 (KfiA) and heparosan synthase-2 (pmHS2) from Pasteurella multocida. ...[more]

PMID: 23524108

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