Ontology highlight
ABSTRACT:
SUBMITTER: Gerety SS
PROVIDER: S-EPMC3640221 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Gerety Sebastian S SS Breau Marie A MA Sasai Noriaki N Xu Qiling Q Briscoe James J Wilkinson David G DG
Development (Cambridge, England) 20130501 10
We have generated an inducible system to control the timing of transgene expression in zebrafish and chick. An estrogen receptor variant (ERT2) fused to the GAL4 transcriptional activator rapidly and robustly activates transcription within 3 hours of treatment with the drug 4-hydroxy-tamoxifen (4-OHT) in tissue culture and transgenic zebrafish. We have generated a broadly expressed inducible ERT2-GAL4 zebrafish line using the ubiquitin (ubi) enhancer. In addition, use of ERT2-GAL4 in conjunction ...[more]