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Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-? and IL-10.


ABSTRACT: This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-? and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-? responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-? responses on PHA-stimulated cells. Interestingly, the IFN-? low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-? responses as well as IL-10 inducing segments with inhibitory capabilities.

SUBMITTER: Burgara-Estrella A 

PROVIDER: S-EPMC3640520 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Predicted peptides from non-structural proteins of porcine reproductive and respiratory syndrome virus are able to induce IFN-γ and IL-10.

Burgara-Estrella Alexel A   Díaz Ivan I   Rodríguez-Gómez Irene M IM   Essler Sabine E SE   Hernández Jesús J   Mateu Enric E  

Viruses 20130211 2


This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened fo  ...[more]

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