Unknown

Dataset Information

0

The human Tim-Tipin complex interacts directly with DNA polymerase epsilon and stimulates its synthetic activity.

ABSTRACT: The Tim-Tipin complex plays an important role in the S phase checkpoint and replication fork stability in metazoans, but the molecular mechanism underlying its biological function is poorly understood. Here, we present evidence that the recombinant human Tim-Tipin complex (and Tim alone) markedly enhances the synthetic activity of DNA polymerase ?. In contrast, no significant effect on the synthetic ability of human DNA polymerase ? and ? by Tim-Tipin was observed. Surface plasmon resonance measurements and co-immunoprecipitation experiments revealed that recombinant DNA polymerase ? directly interacts with either Tim or Tipin. In addition, the results of DNA band shift assays suggest that the Tim-Tipin complex (or Tim alone) is able to associate with DNA polymerase ? bound to a 40-/80-mer DNA ligand. Our results are discussed in view of the molecular dynamics at the human DNA replication fork.

SUBMITTER: Aria V 

PROVIDER: S-EPMC3642320 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

The human Tim-Tipin complex interacts directly with DNA polymerase epsilon and stimulates its synthetic activity.

Aria Valentina V   De Felice Mariarita M   Di Perna Roberta R   Uno Shuji S   Masai Hisao H   Syväoja Juhani E JE   van Loon Barbara B   Hübscher Ulrich U   Pisani Francesca M FM  

The Journal of biological chemistry 20130319 18

The Tim-Tipin complex plays an important role in the S phase checkpoint and replication fork stability in metazoans, but the molecular mechanism underlying its biological function is poorly understood. Here, we present evidence that the recombinant human Tim-Tipin complex (and Tim alone) markedly enhances the synthetic activity of DNA polymerase ε. In contrast, no significant effect on the synthetic ability of human DNA polymerase α and δ by Tim-Tipin was observed. Surface plasmon resonance meas  ...[more]

Similar Datasets

Unknown Human Tim-Tipin complex affects the biochemical properties of the replicative DNA helicase and DNA polymerases.
| S-EPMC3574903 | biostudies-other
Unknown Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in Metazoan DNA Replication.
| S-EPMC7262601 | biostudies-literature
Unknown Knock-down of the TIM/TIPIN complex promotes apoptosis in melanoma cells.
| S-EPMC7244016 | biostudies-literature
Unknown The human papillomavirus DNA helicase E1 binds, stimulates, and confers processivity to cellular DNA polymerase epsilon.
| S-EPMC5758917 | biostudies-literature
Unknown Human DNA polymerase ε is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.
| S-EPMC4917431 | biostudies-literature
Unknown MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon.
| S-EPMC110721 | biostudies-literature
Unknown Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex.
| S-EPMC1876720 | biostudies-literature
Unknown Timeless functions independently of the Tim-Tipin complex to promote sister chromatid cohesion in normal human fibroblasts.
| S-EPMC3127161 | biostudies-literature
Unknown Studies on human DNA polymerase epsilon and GINS complex and their role in DNA replication.
| S-EPMC3190704 | biostudies-literature
Unknown DNA polymerase epsilon: a polymerase of unusual size (and complexity).
| S-EPMC3694787 | biostudies-literature