Dataset Information

C5a receptor deficiency alters energy utilization and fat storage.

ABSTRACT:

Objective

To investigate the impact of whole body C5a receptor (C5aR) deficiency on energy metabolism and fat storage.

Design

Male wildtype (WT) and C5aR knockout (C5aRKO) mice were fed a low fat (CHOW) or a high fat high sucrose diet-induced obesity (DIO) diet for 14 weeks. Body weight and food intake were measured weekly. Indirect calorimetry, dietary fatload clearance, insulin and glucose tolerance tests were also evaluated. Liver, muscle and adipose tissue mRNA gene expression were measured by RT-PCR.

Results

At week one and 12, C5aRKO mice on DIO had increased oxygen consumption. After 12 weeks, although food intake was comparable, C5aRKO mice had lower body weight (-7% CHOW, -12% DIO) as well as smaller gonadal (-38% CHOW, -36% DIO) and inguinal (-29% CHOW, -30% DIO) fat pads than their WT counterparts. Conversely, in WT mice, C5aR was upregulated in DIO vs CHOW diets in gonadal adipose tissue, muscle and liver, while C5L2 mRNA expression was lower in C5aRKO on both diet. Furthermore, blood analysis showed lower plasma triglyceride and non-esterified fatty acid levels in both C5aRKO groups, with faster postprandial triglyceride clearance after a fatload. Additionally, C5aRKO mice showed lower CD36 expression in gonadal and muscle on both diets, while DGAT1 expression was higher in gonadal (CHOW) and liver (CHOW and DIO) and PPAR? was increased in muscle and liver.

Conclusion

These observations point towards a role (either direct or indirect) for C5aR in energy expenditure and fat storage, suggesting a dual role for C5aR in metabolism as well as in immunity.

SUBMITTER: Roy C

PROVIDER: S-EPMC3646841 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

C5a receptor deficiency alters energy utilization and fat storage.

Roy Christian C Gupta Abhishek A Fisette Alexandre A Lapointe Marc M Poursharifi Pegah P Richard Denis D Lu Huiling H Lu Bao B Gerard Norma N Gerard Craig C Cianflone Katherine K

PloS one 20130507 5

<h4>Objective</h4>To investigate the impact of whole body C5a receptor (C5aR) deficiency on energy metabolism and fat storage.<h4>Design</h4>Male wildtype (WT) and C5aR knockout (C5aRKO) mice were fed a low fat (CHOW) or a high fat high sucrose diet-induced obesity (DIO) diet for 14 weeks. Body weight and food intake were measured weekly. Indirect calorimetry, dietary fatload clearance, insulin and glucose tolerance tests were also evaluated. Liver, muscle and adipose tissue mRNA gene expression ...[more]

PMID: 23667486

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Dataset Information

C5a receptor deficiency alters energy utilization and fat storage.

Objective

Design

Results

Conclusion

Publications

C5a receptor deficiency alters energy utilization and fat storage.

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