A novel PCR assay for quantification of HIV-1 RNA.
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ABSTRACT:
SUBMITTER: Shan L
PROVIDER: S-EPMC3648108 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Publications
A novel PCR assay for quantification of HIV-1 RNA.
Shan Liang L Rabi S Alireza SA Laird Gregory M GM Eisele Evelyn E EE Zhang Hao H Margolick Joseph B JB Siliciano Robert F RF
Journal of virology 20130327 11
Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity. ...[more]
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