Synthesis of ¹³C- and ¹?C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies.
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ABSTRACT:
SUBMITTER: Piecyk K
PROVIDER: S-EPMC3652009 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Synthesis of ¹³C- and ¹⁴C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies.
Piecyk Karolina K Davis Richard E RE Jankowska-Anyszka Marzena M
Bioorganic & medicinal chemistry letters 20120504 13
Herein we describe the first simple and short method for specific labeling of mono- and trimethylated dinucleotide mRNA cap analogues with (13)C and (14)C isotopes. The labels were introduced within the cap structures either at the N7 for monomethylguanosine cap or N7 and N2 position for trimethylguanosine cap. The compounds designed for structural and biochemical studies will be useful tools for better understanding the role of the mRNA cap structures in pre-mRNA splicing, nucleocytoplasmic tra ...[more]
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