Ontology highlight
ABSTRACT:
SUBMITTER: Ross W
PROVIDER: S-EPMC3654024 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Ross Wilma W Vrentas Catherine E CE Sanchez-Vazquez Patricia P Gaal Tamas T Gourse Richard L RL
Molecular cell 20130425 3
The global regulatory nucleotide ppGpp ("magic spot") regulates transcription from a large subset of Escherichia coli promoters, illustrating how small molecules can control gene expression promoter-specifically by interacting with RNA polymerase (RNAP) without binding to DNA. However, ppGpp's target site on RNAP, and therefore its mechanism of action, has remained unclear. We report here a binding site for ppGpp on E. coli RNAP, identified by crosslinking, protease mapping, and analysis of muta ...[more]