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Statistical platform to discern spatial and temporal coordination of endothelial sprouting.

ABSTRACT: Many biological processes, including angiogenesis, involve intercellular feedback and temporal coordination, but inference of these relations is often drowned in low sample sizes or noisy population data. To address this issue, a methodology was developed to statistically study spatial lateral inhibition and temporal synchronization in one specific biological process, endothelial sprouting mediated by Notch signaling. Notch plays an essential role in the development of organized vasculature, but the effects of Notch on the temporal characteristics of angiogenesis are not well understood. Results from this study showed that Notch lateral inhibition operates at distances less than 31 ?m. Furthermore, combining time lapse microscopy with an intraclass correlation model typically used to analyze family data showed intrinsic temporal synchronization among endothelial sprouts originating from the same microcarrier. Such synchronization was reduced with Notch inhibitors, but was enhanced with the addition of Notch ligands. These results indicate that Notch plays a critical role in the temporal regulation of angiogenesis, as well as spatial control, and this method of analysis will be of significant utility in studies of a variety of other biological processes.

SUBMITTER: Yuen WW

PROVIDER: S-EPMC3654550 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Statistical platform to discern spatial and temporal coordination of endothelial sprouting.

Yuen William W WW Du Nan R NR Shvartsman Dima D Arany Praveen R PR Lam Henry H Mooney David J DJ

Integrative biology : quantitative biosciences from nano to macro 20120209 3

Many biological processes, including angiogenesis, involve intercellular feedback and temporal coordination, but inference of these relations is often drowned in low sample sizes or noisy population data. To address this issue, a methodology was developed to statistically study spatial lateral inhibition and temporal synchronization in one specific biological process, endothelial sprouting mediated by Notch signaling. Notch plays an essential role in the development of organized vasculature, but ...[more]

PMID: 22318325

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Dataset Information

Statistical platform to discern spatial and temporal coordination of endothelial sprouting.

Publications

Statistical platform to discern spatial and temporal coordination of endothelial sprouting.

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