Molecular identification and characterization of an essential pyruvate transporter from Trypanosoma brucei.
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ABSTRACT:
SUBMITTER: Sanchez MA
PROVIDER: S-EPMC3656298 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Molecular identification and characterization of an essential pyruvate transporter from Trypanosoma brucei.
The Journal of biological chemistry 20130408 20
Pyruvate export is an essential physiological process for the bloodstream form of Trypanosoma brucei as the parasite would otherwise accumulate this end product of glucose metabolism to toxic levels. In the studies reported here, genetic complementation in Saccharomyces cerevisiae has been employed to identify a gene (TbPT0) that encodes this vital pyruvate transporter from T. brucei. Expression of TbPT0 in S. cerevisiae reveals that TbPT0 is a high affinity pyruvate transporter. TbPT0 belongs t ...[more]
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