Structural analysis of HopPmaL reveals the presence of a second adaptor domain common to the HopAB family of Pseudomonas syringae type III effectors.
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ABSTRACT:
SUBMITTER: Singer AU
PROVIDER: S-EPMC3656468 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
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Structural analysis of HopPmaL reveals the presence of a second adaptor domain common to the HopAB family of Pseudomonas syringae type III effectors.
Singer Alex U AU Wu Bin B Yee Adelinda A Houliston Scott S Xu Xiaohui X Cui Hong H Skarina Tatiana T Garcia Maite M Semesi Anthony A Arrowsmith Cheryl H CH Savchenko Alexei A
Biochemistry 20111228 1
HopPmaL is a member of the HopAB family of type III effectors present in the phytopathogen Pseudomonas syringae. Using both X-ray crystallography and solution nuclear magnetic resonance, we demonstrate that HopPmaL contains two structurally homologous yet functionally distinct domains. The N-terminal domain corresponds to the previously described Pto-binding domain, while the previously uncharacterised C-terminal domain spans residues 308-385. While structurally similar, these domains do not sha ...[more]
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