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RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing.

ABSTRACT: We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by comparing two honeybee and several stingless bee samples.

SUBMITTER: Stolle E 

PROVIDER: S-EPMC3656931 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing.

Stolle Eckart E   Moritz Robin F A RF  

PloS one 20130517 5

We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by  ...[more]

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