Dataset Information

ITRAQ reveals candidate pancreatic cancer serum biomarkers: influence of obstructive jaundice on their performance.

ABSTRACT:

Background

The aims of our study were to identify serum biomarkers that distinguish pancreatic cancer (pancreatic ductal adenocarcinoma, PDAC) patients from benign pancreatic disease patients and healthy subjects, and to assess the effects of jaundice on biomarker performance.

Methods

Isobaric tags for relative and absolute quantification were used to compare pooled serum and pancreatic juice samples from a test set of 59 and 25 subjects, respectively. Validation was undertaken in 113 independent subjects.

Results

Candidate proteins Complement C5, inter-?-trypsin inhibitor heavy chain H3, ?1-? glycoprotein and polymeric immunoglobulin receptor were elevated in cancer, as were the reference markers CA19-9 and Reg3A. Biliary obstruction had a significant effect on the performance of the markers, in particular within the PDAC group where the presence of jaundice was associated with a significant increase in the levels of all six proteins (P<0.01). Consequently, in the absence of jaundice, proteins showed reduced sensitivity for PDAC patients over benign subjects and healthy controls (HCs). Similarly, in the presence of jaundice, markers showed reduced specificity for PDAC patients over benign subjects with jaundice. Combining markers enabled improved sensitivity for non-jaundiced PDAC patients over HCs and improved specificity for jaundiced PDAC patients over jaundiced benign disease subjects.

Conclusions

The presence-absence of jaundice in the clinical scenario severely impacts the performance of biomarkers for PDAC diagnosis and has implications for their clinical translation.

SUBMITTER: Tonack S

PROVIDER: S-EPMC3658525 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Publications

iTRAQ reveals candidate pancreatic cancer serum biomarkers: influence of obstructive jaundice on their performance.

Tonack S S Jenkinson C C Cox T T Elliott V V Jenkins R E RE Kitteringham N R NR Greenhalf W W Shaw V V Michalski C W CW Friess H H Neoptolemos J P JP Costello E E

British journal of cancer 20130411 9

<h4>Background</h4>The aims of our study were to identify serum biomarkers that distinguish pancreatic cancer (pancreatic ductal adenocarcinoma, PDAC) patients from benign pancreatic disease patients and healthy subjects, and to assess the effects of jaundice on biomarker performance.<h4>Methods</h4>Isobaric tags for relative and absolute quantification were used to compare pooled serum and pancreatic juice samples from a test set of 59 and 25 subjects, respectively. Validation was undertaken in ...[more]

PMID: 23579209

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