Ontology highlight
ABSTRACT:
SUBMITTER: Dodd DW
PROVIDER: S-EPMC3660070 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Dodd David W DW Gagnon Keith T KT Corey David R DR
Nucleic acid therapeutics 20130508 3
Accurate determination of the amount of a given RNA within a cell is necessary to gain a full understanding of the RNA's function and regulation. Typically, the abundance of RNA is measured by quantitative polymerase chain reaction (qPCR). With qPCR, however, absolute quantification is not possible unless an adequate reference standard curve is generated. The method is not well suited for detecting low copy number templates and values vary depending on the specific primers used. To overcome thes ...[more]