Ontology highlight
ABSTRACT:
SUBMITTER: Sekine H
PROVIDER: S-EPMC3660653 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Sekine Hidekazu H Shimizu Tatsuya T Sakaguchi Katsuhisa K Dobashi Izumi I Wada Masanori M Yamato Masayuki M Kobayashi Eiji E Umezu Mitsuo M Okano Teruo T
Nature communications 20130101
In vitro fabrication of functional vascularized three-dimensional tissues has been a long-standing objective in the field of tissue engineering. Here we report a technique to engineer cardiac tissues with perfusable blood vessels in vitro. Using resected tissue with a connectable artery and vein as a vascular bed, we overlay triple-layer cardiac cell sheets produced from coculture with endothelial cells, and support the tissue construct with media perfused in a bioreactor. We show that endotheli ...[more]