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Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages.

ABSTRACT: Mycobacterium species have developed numerous strategies to avoid the antimycobacterial actions of macrophages, enabling them to survive within the generally inhospitable environment of the cell. The recently identified MSMEG_5817 protein from M. smegmatis is highly conserved in Mycobacterium spp. and is required for bacterial survival in macrophages. Here, the cloning, expression, purification and crystallization of MSMEG_5817 is reported. Crystals of MSMEG_5817 were grown in 1.42?M Li2SO4, 0.1?M Tris-HCl pH 7.7, 0.1?M sodium citrate tribasic dihydrate. Native and multiple-wavelength anomalous dispersion (MAD) data sets have been collected and structure determination is in progress.

SUBMITTER: Shahine AE

PROVIDER: S-EPMC3660903 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages.

Shahine Adam E AE Chan Phooi Y PY Littler Dene D Vivian Julian J Brammananth Rajini R Crellin Paul K PK Coppel Ross L RL Rossjohn Jamie J Beddoe Travis T

Acta crystallographica. Section F, Structural biology and crystallization communications 20130430 Pt 5

Mycobacterium species have developed numerous strategies to avoid the antimycobacterial actions of macrophages, enabling them to survive within the generally inhospitable environment of the cell. The recently identified MSMEG_5817 protein from M. smegmatis is highly conserved in Mycobacterium spp. and is required for bacterial survival in macrophages. Here, the cloning, expression, purification and crystallization of MSMEG_5817 is reported. Crystals of MSMEG_5817 were grown in 1.42 M Li2SO4, 0.1 ...[more]

PMID: 23695579

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