Evaluation of a multiplex assay for estimation of HIV-1 incidence.

ABSTRACT:

Objectives

Accurate methods of estimating HIV-1 incidence are critical for monitoring the status of the epidemic and the impact of prevention strategies. Although several laboratory-based tests have been developed strictly for this purpose, several limitations exist and improved methods or technologies are needed. We sought to further optimize a previously described bead-based, HIV-1-specific multiplex assay with the capability of measuring multiple immune responses for determining recent infection.

Methods

We refined the customized HIV-1 Bio-Plex assay by determining cutoffs and mean durations of recency (MDR), based on the reactivity to longitudinal seroconversion specimens (n = 1347) from 311 ART-naïve, HIV-1-infected subjects. False-recent rates (FRRs) were calculated fo

SUBMITTER: Curtis KA 

PROVIDER: S-EPMC3661489 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature