Microbial translocation is associated with extensive immune activation in dengue virus infected patients with severe disease.

ABSTRACT:

Background

Severe dengue virus (DENV) disease is associated with extensive immune activation, characterized by a cytokine storm. Previously, elevated lipopolysaccharide (LPS) levels in dengue were found to correlate with clinical disease severity. In the present cross-sectional study we identified markers of microbial translocation and immune activation, which are associated with severe manifestations of DENV infection.

Methods

Serum samples from DENV-infected patients were collected during the outbreak in 2010 in the State of São Paulo, Brazil. Levels of LPS, lipopolysaccharide binding protein (LBP), soluble CD14 (sCD14) and IgM and IgG endotoxin core antibodies were determined by ELISA. Thirty cytokines were quantified using a multiplex luminex system. Patients were classi

SUBMITTER: van de Weg CA 

PROVIDER: S-EPMC3662706 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature