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FRET excited ratiometric oxygen sensing in living tissue.

ABSTRACT: Dynamic analysis of oxygen (O?) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O?] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.

SUBMITTER: Ingram JM

PROVIDER: S-EPMC3664065 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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FRET excited ratiometric oxygen sensing in living tissue.

Ingram Justin M JM Zhang Chunfeng C Xu Jian J Schiff Steven J SJ

Journal of neuroscience methods 20130117 1

Dynamic analysis of oxygen (O₂) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glas ...[more]

PMID: 23333398

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