Ontology highlight
ABSTRACT:
SUBMITTER: Lee I
PROVIDER: S-EPMC3664819 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Lee Imsang I Dong Ken C KC Berger James M JM
Nucleic acids research 20130410 10
Type IIA topoisomerases control DNA supercoiling and separate newly replicated chromosomes using a complex DNA strand cleavage and passage mechanism. Structural and biochemical studies have shown that these enzymes sharply bend DNA by as much as 150°; an invariant isoleucine, which has been seen structurally to intercalate between two base pairs outside of the DNA cleavage site, has been suggested to promote deformation. To test this assumption, we examined the role of isoleucine on DNA binding, ...[more]