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Effects of resistin on porcine ovarian follicle steroidogenesis in prepubertal animals: an in vitro study.


ABSTRACT: Resistin was first reported to be an adipocyte-specific hormone, but recent studies have indicated a connection between resistin and reproductive function. However, it is not yet known if resistin is expressed by the ovary and if it can affect steroidogenesis in ovarian follicles from prepubertal pigs.In this study, using real time PCR, immunoblotting, and ELISA, we quantified resistin expression and concentration in maturing ovarian follicles (small, 3-4 mm; medium, 4-5 mm; large, 6-7 mm) collected from prepubertal pigs. In addition, the dose-responsive effects of recombinant human resistin (0.1, 1, 10, and 100 ng/ml) on steroid hormone (i.e., progesterone [P4], androstendione [A4], testosterone [T], and estradiol [E2]) secretion in culture medium and steroidogenic enzyme (i.e., CYP11A1, 3betaHSD, CYP17A1, 17betaHSD, and CYP19A1) expression in ovarian follicles were determined.We observed that resistin gene and protein expression increased significantly (P?

SUBMITTER: Rak-Mardyla A 

PROVIDER: S-EPMC3665660 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Effects of resistin on porcine ovarian follicle steroidogenesis in prepubertal animals: an in vitro study.

Rak-Mardyła Agnieszka A   Durak Martyna M   Lucja Gregoraszczuk Ewa E  

Reproductive biology and endocrinology : RB&E 20130517


<h4>Background</h4>Resistin was first reported to be an adipocyte-specific hormone, but recent studies have indicated a connection between resistin and reproductive function. However, it is not yet known if resistin is expressed by the ovary and if it can affect steroidogenesis in ovarian follicles from prepubertal pigs.<h4>Methods</h4>In this study, using real time PCR, immunoblotting, and ELISA, we quantified resistin expression and concentration in maturing ovarian follicles (small, 3-4 mm; m  ...[more]

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