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Promoter type influences transcriptional topography by targeting genes to distinct nucleoplasmic sites.

ABSTRACT: Both the sequence of a promoter and the position of a gene in 3D nuclear space play crucial roles in gene regulation, but few studies address their inter-relationship. Using human and viral promoters on mini-chromosomes and RNA fluorescence in situ hybridization coupled to 'high-precision' localization, we show that promoters binding the same transcription factors and responding to the same signaling pathways tend to be co-transcribed in the same transcription factories. We go on to suggest how such spatial co-association might drive co-regulation of genes under the control of similar cis-elements.

SUBMITTER: Larkin JD

PROVIDER: S-EPMC3666257 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Promoter type influences transcriptional topography by targeting genes to distinct nucleoplasmic sites.

Larkin Joshua D JD Papantonis Argyris A Cook Peter R PR

Journal of cell science 20130226 Pt 9

Both the sequence of a promoter and the position of a gene in 3D nuclear space play crucial roles in gene regulation, but few studies address their inter-relationship. Using human and viral promoters on mini-chromosomes and RNA fluorescence in situ hybridization coupled to 'high-precision' localization, we show that promoters binding the same transcription factors and responding to the same signaling pathways tend to be co-transcribed in the same transcription factories. We go on to suggest how ...[more]

PMID: 23444365

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