Ontology highlight
ABSTRACT:
SUBMITTER: Lu J
PROVIDER: S-EPMC3666631 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Lu Jiamiao J Zhang Feijie F Kay Mark A MA
Molecular therapy : the journal of the American Society of Gene Therapy 20130305 5
The bacterial backbone (BB) sequences contained within a canonical plasmid DNA dampen exogenous transgene expression by tenfold to 1,000-fold over a period of a few weeks following transfection into quiescent tissues such as the liver. Minicircle DNA vectors devoid of bacterial plasmid backbone sequences overcome transgene silencing providing persistent transgene expression. Because, we recently established that the length rather than sequence of the DNA flanking the transgene expression cassett ...[more]