Dataset Information

Dietary uptake of Wedelia chinensis extract attenuates dextran sulfate sodium-induced colitis in mice.

ABSTRACT:

Scope

Traditional medicinal herbs are increasingly used as alternative therapies in patients with inflammatory diseases. Here we evaluated the effect of Wedelia chinensis, a medicinal herb commonly used in Asia, on the prevention of dextran sulfate sodium (DSS)-induced acute colitis in mice. General safety and the effect of different extraction methods on the bioactivity of W. chinensis were also explored.

Methods and results

C57BL/6 mice were administrated hot water extract of fresh W. chinensis (WCHF) orally for one week followed by drinking water containing 2% DSS for nine days. WCHF significantly attenuated the symptoms of colitis including diarrhea, rectal bleeding and loss of body weight; it also reduced the shortening of colon length and histopathological damage caused by colonic inflammation. Among four W. chinensis extracts prepared using different extraction techniques, WCHF showed the highest anti-colitis efficacy. Analyses of specific T-cell regulatory cytokines (TNF-?, IL-4, IFN-?, IL-17, TGF-?, IL-12) revealed that WCHF treatment can suppress the Th1 and Th17, but not Th2, responses in colon tissues and dendritic cells of DSS-induced colitis mice. A 28-day subacute toxicity study showed that daily oral administration of WCHF (100, 500, 1000 mg/kg body weight) was not toxic to mice.

Conclusion

Together, our findings suggest that specific extracts of W. chinensis have nutritional potential for future development into nutraceuticals or dietary supplements for treatment of inflammatory bowel disease.

SUBMITTER: Huang YT

PROVIDER: S-EPMC3667021 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Dietary uptake of Wedelia chinensis extract attenuates dextran sulfate sodium-induced colitis in mice.

Huang Yuh-Ting YT Wen Chih-Chun CC Chen Yung-Hsiang YH Huang Wen-Ching WC Huang Li-Ting LT Lin Wen-Ching WC Arulselvan Palanisamy P Liao Jiunn-Wang JW Lin Shu-Hui SH Hsiao Pei-Wen PW Kuo Sheng-Chu SC Yang Ning-Sun NS

PloS one 20130529 5

<h4>Scope</h4>Traditional medicinal herbs are increasingly used as alternative therapies in patients with inflammatory diseases. Here we evaluated the effect of Wedelia chinensis, a medicinal herb commonly used in Asia, on the prevention of dextran sulfate sodium (DSS)-induced acute colitis in mice. General safety and the effect of different extraction methods on the bioactivity of W. chinensis were also explored.<h4>Methods and results</h4>C57BL/6 mice were administrated hot water extract of fr ...[more]

PMID: 23734189

Similar Datasets

Project description:IntroductionDietary fiber and flavonoids are promising drugs reported in the treatment of inflammatory bowel disease (IBD). However, it is unclear the interaction between dietary fiber and flavonoids in gut health.ObjectiveThe therapeutic effect of celery, kale, and red chicory powders on colitis mice using non-group feeding cages was investigated. Further, the efficacy of whole celery, celery soluble dietary fiber (CSDF), celery insoluble dietary fiber (CIDF), celery flavonoids (CF), CSDF + CF and CIDF + CF in IBD mice model was assessed to dissect protective effect to attribute to which component(s) in such complex matrix.Methods3% Dextran sulfate sodium salt (DSS) was used to induce mice colitis model. Multiple molecular biological methods were employed to evaluate the severity of mice colitis and the gut microbial composition of mice.ResultsAdministration of kale and red chicory significantly restored body weight, DAI score, and colon length in colonic mice, and celery showed the weakest effects. Administration of either CSDF or CF markedly improved the histological damage, increased colonic mucus expression, and reduced colonic MPO/iNOS activities, and IL-6/IL-1β levels. However, CSDF + CF showed weaker improvement than CF or SDF in most physical and biochemical signs. Furthermore, CSDF and CF decreased intestinal g_Escherichia-Shihella and g_Clostridium_sensu_stricto_1 induced by DSS administration. Interestingly, celery flavonoid promoted g_Akkermansia proliferation both in vivo and in vitro, and which can be inhibited by CSDF.ConclusionsThis study revealed for the first time that CSDF can suppress the protective effect of CF on intestinal health by inhibiting g_Akkermansia, and clarified that the decreased efficacy of celery whole food on colitis was mediated by an antagonism between CSDF and CF. Moreover, this study presents for the first time that interaction between soluble dietary fiber and flavonoids in vivo can ameliorate the efficacy of dietary fiber or flavonoids when administered alone suggestive for an antagonistic effect.

Dataset Information

Dietary uptake of Wedelia chinensis extract attenuates dextran sulfate sodium-induced colitis in mice.

Scope

Methods and results

Conclusion

Publications

Dietary uptake of Wedelia chinensis extract attenuates dextran sulfate sodium-induced colitis in mice.

Similar Datasets

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