Unknown

Dataset Information

0

IFN-? mediates the antitumor effects of radiation therapy in a murine colon tumor.


ABSTRACT: Cancer treatments using ionizing radiation (IR) therapy are thought to act primarily through the induction of tumor cell damage at a molecular level. However, a new concept has recently emerged, suggesting that the immune system is required for effective IR therapy. Our work here has identified interferon gamma (IFN-?) as an essential cytokine for the efficacy of IR therapy. Local IR (15 Gy) to mice bearing Colon38, a colon adenocarcinoma, decreases tumor burden in wild-type animals. Interestingly, IR therapy had no effect on tumor burden in IFN?KO mice. We further determined that intratumoral levels of IFN-? increased 2 days following IR, which directly correlated with a decrease in tumor burden that was not a result of direct cytotoxic effects of IFN-? on tumor cells. T cells from IR-treated tumors exhibited a far greater capacity to lyse tumor cells in a (51)Cr release assay, a process that was dependent on IFN-?. CD8(+) T cells were the predominant producers of IFN-?, as demonstrated by IFN-? intracellular staining and studies in IFN-? reporter mice. Elimination of CD8(+) T cells by antibody treatment reduced the intratumoral levels of IFN-? by over 90%. More importantly, elimination of CD8(+) T cells completely abrogated the effects of radiation therapy. Our data suggest that IFN-? plays a pivotal role in mediating the antitumor effects of IR therapy.

SUBMITTER: Gerber SA 

PROVIDER: S-EPMC3668027 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

IFN-γ mediates the antitumor effects of radiation therapy in a murine colon tumor.

Gerber Scott A SA   Sedlacek Abigail L AL   Cron Kyle R KR   Murphy Shawn P SP   Frelinger John G JG   Lord Edith M EM  

The American journal of pathology 20130412 6


Cancer treatments using ionizing radiation (IR) therapy are thought to act primarily through the induction of tumor cell damage at a molecular level. However, a new concept has recently emerged, suggesting that the immune system is required for effective IR therapy. Our work here has identified interferon gamma (IFN-γ) as an essential cytokine for the efficacy of IR therapy. Local IR (15 Gy) to mice bearing Colon38, a colon adenocarcinoma, decreases tumor burden in wild-type animals. Interesting  ...[more]

Similar Datasets

| S-EPMC9273087 | biostudies-literature
| S-EPMC4934370 | biostudies-literature
| S-EPMC10510866 | biostudies-literature
| S-EPMC9564135 | biostudies-literature
| S-EPMC7156830 | biostudies-literature
| S-EPMC9757747 | biostudies-literature
| S-EPMC7011019 | biostudies-literature
| S-EPMC6625917 | biostudies-literature
| S-EPMC1544164 | biostudies-literature
| S-EPMC5564749 | biostudies-literature