Ontology highlight
ABSTRACT:
SUBMITTER: Tanaka N
PROVIDER: S-EPMC3668522 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Tanaka Natsuki N Kawachi Miki M Fujiwara Takashi T Maeshima Masayoshi M
FEBS open bio 20130423
The vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, AtMTP1, has a cytosolic histidine-rich loop (His-loop). We characterized the structures and Zn(2+)-binding properties of the His-loop and other domains. Circular dichroism analyses revealed that the His-loop partly consists of a polyproline type II structure and that its conformational change is induced by Zn(2+) as well as the C-terminal domain. Isothermal titration calorimetry of the His-loop revealed a binding number of four Zn(2+) ...[more]