Ontology highlight
ABSTRACT:
SUBMITTER: Zhang X
PROVIDER: S-EPMC3670040 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Zhang Xuxiao X Vadas Oscar O Perisic Olga O Anderson Karen E KE Clark Jonathan J Hawkins Phillip T PT Stephens Len R LR Williams Roger L RL
Molecular cell 20110301 5
Phosphoinositide 3-kinases (PI3Ks) are essential for cell growth, migration, and survival. The structure of a p110β/p85β complex identifies an inhibitory function for the C-terminal SH2 domain (cSH2) of the p85 regulatory subunit. Mutagenesis of a cSH2 contact residue activates downstream signaling in cells. This inhibitory contact ties up the C-terminal region of the p110β catalytic subunit, which is essential for lipid kinase activity. In vitro, p110β basal activity is tightly restrained by co ...[more]