In vivo detection and quantification of chemicals that enhance protein stability.
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ABSTRACT:
SUBMITTER: Hailu TT
PROVIDER: S-EPMC3670414 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
In vivo detection and quantification of chemicals that enhance protein stability.
Hailu Tsinatkeab T TT Foit Linda L Bardwell James C A JC
Analytical biochemistry 20121205 1
We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability. ...[more]
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