New tools for the identification of developmentally regulated enhancer regions in embryonic and adult zebrafish.
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ABSTRACT:
SUBMITTER: Levesque MP
PROVIDER: S-EPMC3670562 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
New tools for the identification of developmentally regulated enhancer regions in embryonic and adult zebrafish.
Levesque Mitchell P MP Krauss Jana J Koehler Carla C Boden Cindy C Harris Matthew P MP
Zebrafish 20130305 1
We have conducted a screen to identify developmentally regulated enhancers that drive tissue-specific Gal4 expression in zebrafish. We obtained 63 stable transgenic lines with expression patterns in embryonic or adult zebrafish. The use of a newly identified minimal promoter from the medaka edar locus resulted in a relatively unbiased set of expression patterns representing many tissue types derived from all germ layers. Subsequent detailed characterization of selected lines showed strong and re ...[more]
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