A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages.
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ABSTRACT:
SUBMITTER: Costa-Nunes JA
PROVIDER: S-EPMC3671114 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Publications
A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages.
Costa-Nunes José António da JA
SpringerPlus 20130524 1
Here is reported a novel histological marker line that strongly and specifically labels the male gametophyte in Arabidopsis flowers, from the uninucleate microspore to the pollination stage. In this marker line, the expression of the UidA and GFP marker genes is driven by the promoter of the At5g17340 Arabidopsis gene, a gene highly expressed in the male gametophyte. Strong GUS histochemical staining is detected in the uninucleate microspores. Strong GUS, and GFP, histological labelling is also ...[more]
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