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Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam.

ABSTRACT: Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal RNA revealed that 13 out of 16 isolates were of Fasciola gigantica type, whereas three isolates presented a hybrid sequence from F. gigantica and Fasciola hepatica. Interestingly, all the mitochondrial sequences (partial COI and NDI) were of F. gigantica type, suggesting that the maternal lineage of the hybrid form is from F. gigantica. No intra-sequence variation was detected.

SUBMITTER: Nguyen S

PROVIDER: S-EPMC3671424 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam.

Nguyen S S Amer S S Ichikawa M M Itagaki T T Fukuda Y Y Nakai Y Y

Parasite (Paris, France) 20120201 1

Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal RNA revealed that 13 out of 16 isolates were of Fasciola gigantica type, whereas three isolates presen ...[more]

PMID: 22314245

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Molecular identification of Fasciola spp. (Digenea: Fasciolidae) in Egypt.

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Molecular Characterization of Animal Fasciola Spp. Isolates from Lorestan Province, Western Iran.

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Genotyping and Phylogenetic Analysis of Fasciola Spp. Isolated from Sheep and Cattle Using PCR-RFLP in Ardabil Province, Northwestern Iran.

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Is Galba schirazensis (Mollusca, Gastropoda) an intermediate host of Fasciola hepatica (Trematoda, Digenea) in Ecuador?

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| S-EPMC5492793 | biostudies-literature

Molecular identification of Sarcocystis spp. in cattle: partial sequencing of Cytochrome C Oxidase subunit 1 (COI).

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