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Simultaneous detection of group a rotavirus in Swine and rat on a pig farm in Brazil.

ABSTRACT: This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil.

SUBMITTER: Tonietti Pde O

PROVIDER: S-EPMC3671536 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Simultaneous detection of group a rotavirus in Swine and rat on a pig farm in Brazil.

Tonietti Paloma de Oliveira Pde O da Hora Aline Santana AS Silva Fernanda Dornellas F FD Ferrari Karen Linares KL Brandão Paulo Eduardo PE Richtzenhain Leonardo José LJ Gregori Fabio F

TheScientificWorldJournal 20130516

This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results poi ...[more]

PMID: 23766702

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