Ontology highlight
ABSTRACT:
SUBMITTER: Drukker M
PROVIDER: S-EPMC3672406 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Drukker Micha M Tang Chad C Ardehali Reza R Rinkevich Yuval Y Seita Jun J Lee Andrew S AS Mosley Adriane R AR Weissman Irving L IL Soen Yoav Y
Nature biotechnology 20120527 6
To identify early populations of committed progenitors derived from human embryonic stem cells (hESCs), we screened self-renewing, BMP4-treated and retinoic acid-treated cultures with >400 antibodies recognizing cell-surface antigens. Sorting of >30 subpopulations followed by transcriptional analysis of developmental genes identified four distinct candidate progenitor groups. Subsets detected in self-renewing cultures, including CXCR4(+) cells, expressed primitive endoderm genes. Expression of C ...[more]