Genetic and non-genetic predictors of LINE-1 methylation in leukocyte DNA.

ABSTRACT:

Background

Altered DNA methylation has been associated with various diseases.

Objective

We evaluated the association between levels of methylation in leukocyte DNA at long interspersed nuclear element 1 (LINE-1) and genetic and non-genetic characteristics of 892 control participants from the Spanish Bladder Cancer/EPICURO study.

Methods

We determined LINE-1 methylation levels by pyrosequencing. Individual data included demographics, smoking status, nutrient intake, toenail concentrations of 12 trace elements, xenobiotic metabolism gene variants, and 515 polymorphisms among 24 genes in the one-carbon metabolism pathway. To assess the association between LINE-1 methylation levels (percentage of methylated cytosines) and potential determinants, we estimated beta coeffici

SUBMITTER: Tajuddin SM 

PROVIDER: S-EPMC3672919 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature