Characterization of enteric neurons in wild-type and mutant zebrafish using semi-automated cell counting and co-expression analysis.
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ABSTRACT:
SUBMITTER: Simonson LW
PROVIDER: S-EPMC3673588 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Publications
Characterization of enteric neurons in wild-type and mutant zebrafish using semi-automated cell counting and co-expression analysis.
Simonson Levi W LW Ganz Julia J Melancon Ellie E Eisen Judith S JS
Zebrafish 20130108 2
To characterize fluorescent enteric neurons labeled for expression of cytoplasmic markers in zebrafish mutants, we developed a new MATLAB-based program that can be trained by user input. We used the program to count enteric neurons and to analyze co-expression of the neuronal marker, Elavl, and the neuronal subtype marker, serotonin, in 3D confocal image stacks of dissected whole-mount zebrafish intestines. We quantified the entire population of enteric neurons and the serotonergic subpopulation ...[more]
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