Unknown

Dataset Information

0

MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3.

ABSTRACT: MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress.

SUBMITTER: Sanchez N 

PROVIDER: S-EPMC3675065 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3.

Sanchez Noelia N   Gallagher Mark M   Lao Nga N   Gallagher Clair C   Clarke Colin C   Doolan Padraig P   Aherne Sinead S   Blanco Alfonso A   Meleady Paula P   Clynes Martin M   Barron Niall N  

PloS one 20130606 6

MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti  ...[more]

Similar Datasets

Unknown Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.
| S-EPMC2343599 | biostudies-literature
Unknown S-adenosylmethionine limitation induces p38 mitogen-activated protein kinase and triggers cell cycle arrest in G1.
| S-EPMC3874783 | biostudies-literature
Unknown miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes.
| S-EPMC2532718 | biostudies-literature
Unknown Fbxl12 triggers G1 arrest by mediating degradation of calmodulin kinase I.
| S-EPMC3905573 | biostudies-literature
Unknown The RNA-binding protein SART3 promotes miR-34a biogenesis and G1 cell cycle arrest in lung cancer cells.
| S-EPMC6873168 | biostudies-literature
Unknown MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma.
| S-EPMC3334515 | biostudies-literature
Unknown Involvement of miR-15a in G0/G1 Phase Cell Cycle Arrest Induced by Porcine Circovirus Type 2 Replication.
| S-EPMC4908419 | biostudies-other
Unknown The miR-17-5p microRNA is a key regulator of the G1/S phase cell cycle transition.
| S-EPMC2575517 | biostudies-literature
Unknown The p27-Skp2 axis mediates glucocorticoid-induced cell cycle arrest in T-lymphoma cells.
| S-EPMC3865052 | biostudies-literature
Unknown ZNF674-AS1 antagonizes miR-423-3p to induce G0/G1 cell cycle arrest in non-small cell lung cancer cells.
| S-EPMC7901084 | biostudies-literature