Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.

ABSTRACT: Responses to many growth and stress conditions are assumed to act via changes to the cellular redox status. However, direct measurement of pH-adjusted redox state during growth and stress has never been carried out. Organellar redox state (E GSH) was measured using the fluorescent probes roGFP2 and pHluorin in Saccharomyces cerevisiae. In particular, we investigated changes in organellar redox state in response to various growth and stress conditions to better understand the relationship between redox-, oxidative- and environmental stress response systems. E GSH values of the cytosol, mitochondrial matrix and peroxisome were determined in exponential and stationary phase in various media. These values (-340 to -350 mV) were more reducing than previously reported. Interestingly, sub-cellula

SUBMITTER: Ayer A 

PROVIDER: S-EPMC3676407 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature