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QuaNCAT: quantitating proteome dynamics in primary cells.

ABSTRACT: Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

SUBMITTER: Howden AJ

PROVIDER: S-EPMC3676679 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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QuaNCAT: quantitating proteome dynamics in primary cells.

Howden Andrew J M AJ Geoghegan Vincent V Katsch Kristin K Efstathiou Georgios G Bhushan Bhaskar B Bhushan Bhaskar B Boutureira Omar O Thomas Benjamin B Trudgian David C DC Kessler Benedikt M BM Dieterich Daniela C DC Davis Benjamin G BG Acuto Oreste O

Nature methods 20130310 4

Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activat ...[more]

PMID: 23474466

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