Ontology highlight
ABSTRACT:
SUBMITTER: Hafner M
PROVIDER: S-EPMC3677277 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Hafner Markus M Max Klaas E A KE Bandaru Pradeep P Morozov Pavel P Gerstberger Stefanie S Brown Miguel M Molina Henrik H Tuschl Thomas T
RNA (New York, N.Y.) 20130312 5
Human LIN28A and LIN28B are RNA-binding proteins (RBPs) conserved in animals with important roles during development and stem cell reprogramming. We used Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) in HEK293 cells and identified a largely overlapping set of ∼3000 mRNAs at ∼9500 sites located in the 3' UTR and CDS. In vitro and in vivo, LIN28 preferentially bound single-stranded RNA containing a uridine-rich element and one or more flanking guanosines ...[more]