Unknown

Dataset Information

0

GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.


ABSTRACT: GPR37 (also known as Pael-R) and GPR37L1 are orphan G protein-coupled receptors that are almost exclusively expressed in the nervous system. We screened these receptors for potential activation by various orphan neuropeptides, and these screens yielded a single positive hit: prosaptide, which promoted the endocytosis of GPR37 and GPR37L1, bound to both receptors and activated signaling in a GPR37- and GPR37L1-dependent manner. Prosaptide stimulation of cells transfected with GPR37 or GPR37L1 induced the phosphorylation of ERK in a pertussis toxin-sensitive manner, stimulated (35)S-GTP?S binding, and promoted the inhibition of forskolin-stimulated cAMP production. Because prosaptide is the active fragment of the secreted neuroprotective and glioprotective factor prosaposin (also known as sulfated glycoprotein-1), we purified full-length prosaposin and found that it also stimulated GPR37 and GPR37L1 signaling. Moreover, both prosaptide and prosaposin were found to protect primary astrocytes against oxidative stress, with these protective effects being attenuated by siRNA-mediated knockdown of endogenous astrocytic GPR37 or GPR37L1. These data reveal that GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.

SUBMITTER: Meyer RC 

PROVIDER: S-EPMC3677493 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.

Meyer Rebecca C RC   Giddens Michelle M MM   Schaefer Stacy A SA   Hall Randy A RA  

Proceedings of the National Academy of Sciences of the United States of America 20130520 23


GPR37 (also known as Pael-R) and GPR37L1 are orphan G protein-coupled receptors that are almost exclusively expressed in the nervous system. We screened these receptors for potential activation by various orphan neuropeptides, and these screens yielded a single positive hit: prosaptide, which promoted the endocytosis of GPR37 and GPR37L1, bound to both receptors and activated signaling in a GPR37- and GPR37L1-dependent manner. Prosaptide stimulation of cells transfected with GPR37 or GPR37L1 ind  ...[more]

Similar Datasets

| S-EPMC6492175 | biostudies-literature
| S-EPMC7707515 | biostudies-literature
| S-EPMC8357083 | biostudies-literature
| S-EPMC7063848 | biostudies-literature
| S-EPMC9144339 | biostudies-literature
| S-EPMC6084904 | biostudies-literature
| S-EPMC8369841 | biostudies-literature
| S-EPMC5569905 | biostudies-literature
2020-01-13 | PXD015202 | Pride
| S-EPMC3503176 | biostudies-literature