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Structure of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ?KZ.


ABSTRACT: Pseudomonas ?KZ-like bacteriophages encode a group of related tubulin/FtsZ-like proteins believed to be essential for the correct centring of replicated bacteriophage virions within the bacterial host. In this study, we present crystal structures of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ?KZ in both the monomeric and protofilament states, revealing that ?KZ TubZ undergoes structural changes required to polymerise, forming a canonical tubulin/FtsZ-like protofilament. Combining our structures with previous work, we propose a polymerisation-depolymerisation cycle for the Pseudomonas bacteriophage subgroup of tubulin/FtsZ-like proteins. Electron cryo-microscopy of ?KZ TubZ filaments polymerised in vitro implies a long-pitch helical arrangement for the constituent protofilaments. Intriguingly, this feature is shared by the other known subgroup of bacteriophage tubulin/FtsZ-like proteins from Clostridium species, which are thought to be involved in partitioning the genomes of bacteriophages adopting a pseudo-lysogenic life cycle.

SUBMITTER: Aylett CH 

PROVIDER: S-EPMC3678025 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Structure of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ΦKZ.

Aylett Christopher H S CH   Izoré Thierry T   Amos Linda A LA   Löwe Jan J  

Journal of molecular biology 20130322 12


Pseudomonas ΦKZ-like bacteriophages encode a group of related tubulin/FtsZ-like proteins believed to be essential for the correct centring of replicated bacteriophage virions within the bacterial host. In this study, we present crystal structures of the tubulin/FtsZ-like protein TubZ from Pseudomonas bacteriophage ΦKZ in both the monomeric and protofilament states, revealing that ΦKZ TubZ undergoes structural changes required to polymerise, forming a canonical tubulin/FtsZ-like protofilament. Co  ...[more]

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