Unknown

Dataset Information

0

Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel.


ABSTRACT: Preribosomal particles evolve in the nucleus through transient interaction with biogenesis factors before export to the cytoplasm. Here, we report the architecture of the late pre-60S particle, purified from Saccharomyces cerevisiae, through Arx1, a nuclear export factor with structural homology to methionine aminopeptidases, or its binding partner Alb1. Cryo-EM reconstruction of the Arx1 particle at 11.9-Å resolution reveals regions of extra density on the pre-60S particle attributed to associated biogenesis factors, confirming the immature state of the nascent subunit. One of these densities could be unambiguously assigned to Arx1. Immunoelectron microscopy and UV cross-linking localize Arx1 close to the ribosomal exit tunnel, in direct contact with ES27, a highly dynamic eukaryotic rRNA expansion segment. The binding of Arx1 at the exit tunnel may position this export factor to prevent premature recruitment of ribosome-associated factors active during translation.

SUBMITTER: Bradatsch B 

PROVIDER: S-EPMC3678077 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel.

Bradatsch Bettina B   Leidig Christoph C   Granneman Sander S   Gnädig Marén M   Tollervey David D   Böttcher Bettina B   Beckmann Roland R   Hurt Ed E  

Nature structural & molecular biology 20121111 12


Preribosomal particles evolve in the nucleus through transient interaction with biogenesis factors before export to the cytoplasm. Here, we report the architecture of the late pre-60S particle, purified from Saccharomyces cerevisiae, through Arx1, a nuclear export factor with structural homology to methionine aminopeptidases, or its binding partner Alb1. Cryo-EM reconstruction of the Arx1 particle at 11.9-Å resolution reveals regions of extra density on the pre-60S particle attributed to associa  ...[more]

Similar Datasets

| S-EPMC7363849 | biostudies-literature
| S-EPMC100262 | biostudies-literature
| S-EPMC6118127 | biostudies-literature
| S-EPMC156746 | biostudies-literature
| S-EPMC2620753 | biostudies-literature
| S-EPMC8338941 | biostudies-literature
| S-EPMC3542530 | biostudies-literature
| S-EPMC3783196 | biostudies-literature
| S-EPMC125552 | biostudies-literature
2021-06-25 | GSE174587 | GEO